28S D2-D7 region
While we prefer single-copy, protein-coding genes for higher-level bee phylogeny, regions of the 28S D2-D3 region have been used in bees (Cameron & Mardulyn 2001). We have successfully amplified and sequenced the same region used by Cameron & Mardulyn (2001) for a broad survey of ST and LT bees. Our data set and the one by Cameron & Mardulyn (2001) were generated using primers D2-3665F (Bel28S) and D3-4283R (Mar28Srev) (see primer lists and maps in the attached pdf files below). The resulting alignment has some highly conserved regions, but several expansion regions (loops) make the alignment ambiguous. Virtually all the primer pairs listed in the attached pdf file amplify some or all bees tested. The complete honey bee 28S sequence is available on GenBank as accession number AF181590.
Below is a figure, scanned from Caterino et al. 2000, which shows a map of ribosomal DNA and datasets including 28S sequences that were published prior to March 15, 1999.
Download
Gene Information: |
Map of 28S gene (.pdf file) |
| Related Papers: |